Hieff master mix
Web2. Prepare a master mix for n + 1 samples. 3. Example of master mix for 10 samples: Water,* nuclease-free (Cat. No. R0581) (10 + 1) x 15 µL = 165 µL 10X FastDigest Green Buffer or 10X FastDigest Buffer (10 + 1) x 2 µL = 22 µL FastDigest enzyme (10 + 1) x 1 µL = 11 µL 4. Add 18 μL of master mix* to tubes containing DNA. WebHieff® qPCR SYBR Green Master Mix(No Rox)是2×实时定量PCR扩增的预混合溶液。Mix中含有热启动Hieff® DNA Polymerase、SYBR Green I、dNTPs、Mg2+。使用时， …
Hieff master mix
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WebTable 1. Recommended Master Mix. Reagent Volume Final Conc. CloneAmp HiFi PCR Premix Primer 1 Primer 2 Template Sterilized distilled water 12.5 µl 5–7.5 pmol 5–7.5 pmol < 100 ng up to 25 µl 1X 0.2–0.3 µM 0.2–0.3 µM Total volume per reaction 25.0 µl 2. Mix the Master Mix by tapping the bottom of the tube, then centrifuge briefly.
Web图 1. 2×Hieff TM PCR Master Mix 判定基因敲除小鼠基因型。 与同类品牌 T 产品在相同体系下扩增目的基因， Hieff TM Mix 扩增效率明显较高，结果更明确。 单一条带（ 825bp … WebHotStarTaq Master Mix Kit (250 units) (1000 units) (2500 units) Catalog no. 203443 203445 203446 HotStarTaq Master Mix† 3 x 0.85 ml 12 x 0.85 ml 1 x 25 ml RNase-Free Water 2 x 1.7 ml 8 x 1.7 ml 2 x 20 ml Handbook 1 1 1 HotStarTaq DNA (250 units) (1000 units) (5000 units) (25000 units) Polymerase Catalog no. 203203 203205 203207 203209
WebMix thoroughly, centrifuge briefly to collect solutions at the bottom of tubes, and then store on ice protected from light. 2. Prepare (on ice or at room temperature) enough assay master mix for all reactions by adding all required components, except the DNA template, according to the recommendations in Table 1. Table 1. Reaction Setup* Component WebThe Q5 High-Fidelity 2X Master Mix offers robust, high-fidelity performance in a convenient master mix format. The Q5 High- Fidelity 2X Master Mix features a high-fidelity, …
Web产品描述 Hieff® qPCR SYBR Green Master Mix(No Rox)是2×实时定量PCR扩增的预混合溶液。Mix中含有热启动Hieff ® DNA Polymerase、SYBR Green I、dNTPs、Mg 2+ 。 使用时，仅需在扩增体系中加入模板和引物即可进行实时荧光定量PCR，大大简化操作过程，降低 …
WebThe master mixes greatly simplify your experiment setup as they contain everything you need: buffer, dNTPs, thermostable hot-start DNA polymerase, and, of course, SYBR … diameter of h atomWeb本产品适用于多重PCR实验。Hieff® Multiplex PCR Master Mix为多重PCR酶制剂与扩增缓冲液的2×预混液，可以快速便捷地用于PCR反应，具备高均一性、高特异性和高灵敏度的特点，可以对20对及以上的引物进行特异、均一地进行扩增反应。 circle dishwasherWeb2×Hieff™ PCR Master Mix contains Hieff™ Taq DNA Polymerase (Cat#10101), dNTPs, and other PCR-required components. The Master Mix is stable for 3 months at 4°C with … circle divers sharmWeb本产品适用于多重PCR实验。Hieff® Multiplex PCR Master Mix为多重PCR酶制剂与扩增缓冲液的2×预混液，可以快速便捷地用于PCR反应，具备高均一性、高特异性和高灵敏度 … diameter of haumea in kmWebArraystar SYBR® Green qPCR Master Mix contains all the components for an optimized qPCR reaction, with high stability, excellent repeatability ... Applications: ... 2×Hieff™ … circle divided by 16WebHigh-fidelity PCR enzymes are used for applications requiring high accuracy during DNA amplification such as cloning, sequencing or mutagenesis. Thermo Scientific Phusion high-fidelity DNA polymerases were created by fusing a dsDNA-binding domain to a Pyrococcus polymerase–like proofreading enzyme. Due to this unique protein fusion ... diameter of hair in micronsWeb2×Hieff® PCR Master Mix是即用型的常规PCR预混合溶液，含有Taq DNA Polymerase、dNTP混合物、MgCl2以及优化的缓冲体系。反应时只需加入引物和模板即可进行扩增， … diameter of hela cell