WebProduct Specific Information. MA1-516 detects hypoxia-inducible factor 1 alpha (HIF-1 alpha) from human, non-human primate, bovine, mouse and porcine cells. This antibody does not cross-react with ARNT or the related HIF-2 alpha. MA1-516 has been successfully used in Western blot, immunofluorescence, immunoprecipitation, immunocytochemistry ... Web9 de jun. de 2024 · Chromatin was diluted and input chromatin was collected. Remaining chromatin was used for ChIP by adding 4 μg mouse anti–HIF-1α (Abcam; ab1) as the antibody of interest, 4 μg mouse anti-histone H3 (Abcam, ab1220) as a positive control, and mouse anti-GFP antibody (Abcam, ab1218) as a negative control. Antibodies were …
Hypoxia Pathway Antibody Sampler Kit Cell Signaling …
WebSummary. HIF1 alpha antibody recognizes HIF1 alpha (HIF1a) protein, which is a subunit of the heterodimeric transcription factor hypoxia … WebHIF-1α, NF-κB, and iNOS were measured by immunofluorescence of aortic tissues that were previously fixed with PHEMO buffer (68 mM PIPES, 25 mM, HEPES, pH 6.9, 15 mM EGTA, 3 mM MgCl2, 10% [v/v] dimethyl sulfoxide containing 3.7% formaldehyde and 0.05% glutaraldehyde) and were processed by imumunofluoresence double labeling with anti … impact of food miles on the environment
Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody AF1935: R&D …
WebWestern blot - HIF1α Rabbit pAb (A11945) Western blot analysis of extracts of HeLa cells, using HIF1α antibody (A11945) at 1:1000 dilution.Hela cells were treated by Cobalt chloride (0.1 mM) at 37℃ for 4 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. WebAnti-HIF-1 alpha antibody [EPR16897] (ab179483) Research with confidence – consistent and reproducible results with every batch Long-term and scalable supply – powered by … WebAnti-HIF-1α Antibody is a Rabbit Polyclonal Antibody for detection of HIF-1 alpha also known as ARNT interacting protein or HIF-1 alpha & has been validated in WB. - Find MSDS or SDS, a COA, data sheets and more information. impact of food waste in japan