WebFeb 13, 2016 · Anti-inflammatory bioassay. RAW-Blue cells (5 × 10 4 /well) were cultured in 96-well plates for 24 h, and then treated with LPS (1 μg/mL) alone or together with test samples for 20 h. Secreted embryonic alkaline phosphatase (SEAP) activity in the medium was determined by QUANTI-Blue medium following the manufacturer’s instructions. WebAug 24, 2010 · RAW-Blue cells are derived from RAW-264.7 macrophages that stably express a secreted embroyonic alkaline phosphotase (SEAP) gene which can be induced by NF-κB or AP-1 transcription factors. Treatment of RAW-Blue cells with Rx inhibited the LPS-induced NF-κB activity in a time- and dose-dependent manner as indicated by the reduced …
A novel coumarin, (+)-3′-angeloxyloxy-4′-keto-3′,4′-dihydroseselin ...
WebNF-κB activity from LPS-activated RAW-Blue™ cells after incubation with 30 and 300 μg/ml of acacia (AG), 30 and 300 μg/ml of guar gum (GG) and 30 μg/ml of 5ASA for 24 h in serum-free medium ... WebApr 6, 2024 · RAW 264.7 was inoculated onto a 96-well plate at a density of 2x10^4 cells/well, incubated for 24 h under the above conditions. Then, I stimulated RAW with LPS (1 ug/ml) after 1 hour of drug ... chipset sis
Frontiers Detection of innate immune response modulating …
WebFeb 17, 2024 · Coculture D1 Cells:RAW-Blue Cells Additionally, we prepared cocultures of D1 and RAW-blue cells using transwell inserts without the Dex P-A hydrogel. After 24 h incubation at 37 °C, NF-κB/AP-1 activation upon the previously mentioned stimuli was assessed by measuring the release of secreted embryonic alkaline phosphates (SEAPs) … WebJan 21, 2024 · Raw-blue™ cells were cultured at 37 °C using manufacturer recommendations (InvivoGen) in a 24-well microplate until 70–80% confluence (1 × 10 5 cells/well) was reached. This cell line expressed the secreted embryonic alkaline phosphatase (SEAP) gene under the control of a promoter inducible by the transcription … WebRAW-blue cells were pretreated with 200μM Tamiflu, 100μg/mL MMP9i, or 100μg/mL BIM-23127 for 30min followed by 10μg/mL imiquimod or left cultured in media. Cells (1×107 cells) are pelleted ... chipsets intel®